HEMATOPOIESIS AND STEM CELLS Forward RNAi screens in primary human hematopoietic stem/progenitor cells
نویسندگان
چکیده
1Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston; 2Harvard Stem Cell Institute, Harvard University, Cambridge, MA; 3Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, Lund, Sweden; 4Howard Hughes Medical Institute and Department of Genetics, Center for Genetics and Genomics, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA; 5Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge; 6Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Harvard Medical School, Boston; and 7Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA
منابع مشابه
Forward RNAi screens in primary human hematopoietic stem/progenitor cells.
The mechanisms regulating key fate decisions such as self-renewal and differentiation in hematopoietic stem and progenitor cells (HSPC) remain poorly understood. We report here a screening strategy developed to assess modulators of human hematopoiesis using a lentiviral short hairpin RNA (shRNA) library transduced into cord blood-derived stem/progenitor cells. To screen for modifiers of self-re...
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تاریخ انتشار 2009